THE JOES:
General Hawk - played by Dennis Quaid. because of his great intilligence he graduated top of the class. The leader of the Joes. He is in the military before who leads the best team in the battle field. He receive so many awards and merits.
Duke - played by Channing Tatum. The leader of the military team who were supposed to deliver the nanotechnology to NATO. Duke wants action thats why he always refuse to his friends invitation on joining the airforce. Four years ago he was engage to Ana Lewis before he blamed himself for the killing of Ana's brother Rex Lewis on a mission. He then become one of the Joes when he told the General that he knew the leader of the team who ambushed them.
Scarlett - played by Rachel Nicholas. A very smart lady who graduated college as early as the age of twelve. She did not understand why men are so attracted to her. Later in the story she discover the soft part in her and being in loved with Rip Cord.
Snake Eyes - portrayed by Ray Park and Leo Howard , the 10 year old snake eyes. A ninja commando who took a vow of silence after he has seen his martial art teacher being killed.
Rip Cord - played by Marlon Wayans. A funny man who is a member of Duke's team on deliviring the warhead. He has a passion on flying aircrafts thats why he is convincing Duke his military buddy and bestrfiend that they may join the airforce. He too become a member of the Joes for his extraordinary shooting ability. He shows his affection to his co Joe and the only lady of the team Scarlett. He was responsible for the stopping of the two warhead missile.
Heavy Duty - played by Adewale Akinnuoye-Agbaje. A big black man who is the teams leader of the Joes. responsible for leading the Joe force in combat between the cobra force. Known as the man with out fear.
Breaker - played by Said Taghmaoui. The team's communication specialist and who is always incharge on the programming thing on locating the nanotechnology and the base of MARS. Best known for his love of chewing gum especially during rough minutes. he can also speak seven langguages.
THE VILLAINS :
James McCullen / Destro - played by Christopher Eccleston. The main villain of the film who is the founder and leader of the Military Armament Research Syndicate (MARS). He was the descendant of the McCullen family the biggest arm dealer of France, whose ancestor was accused of betraying the country. His greatest dream is to change the world using the nanotechnology. He took a burn after a physical encounter with duke and the doctor inject him with nanomite incasing his face with a silver.
The Doctor/Cobra Commander/Rex Lewis - played by Joseph Gordon-Levitt. He was Ana Lewis brother and was a member of the military four years ago. Suspected of being killed on a mission headed by duke. In the long run it turn out that rex was alive and assume the identity of the The Doctor who is the mind behind the nanomite technology. He became Cobra commander after implanting a nanomite to James McCullen.
Ana Lewis/ Anastacia de Cobray/ The Baroness - played by Sienna Miller. Four years ago she was engaged to Duke and then they broke up after what happened to his brother Rex. She was implanted by a nanomite and became the memeber of the Cobra and the lover of James McCullen. She became the Baroness when he got married to The Baron de Cobray for some hidden agenda by the cobra.
Thomas Arashikage / Storm Shadow - portrayed by Lee Byung-hun and Brandon Soo Hoo as the young storm shadow. A member of the arashikage clan and the rival of Snake Eyes. When he was 10 he was seen beside the body of his master that is killed.
Zartan - portrayed by Arnold Vosloo. He is serving Destro and was implanted by a nanomite. At the last part of the movie he infiltrate the white house and assumes the identity of the president of the United States.